The procedure starts with plating 0.1 ml of dilutions of heat-treated soil suspensions on glucose mineral base agar : 10g Glc; 1g ( NH 4 ) 2 SO 4 or KNO 3; 0.8g K 2 HPO 4; 0.2g KH 2 PO 4; 0.5g MgSO 4 ?H 2 O; 0.05g CaSO 4 ?H 2 O; 0.01g FeSO 4 ?H 2 O; 12g agar; distilled water to 1 litre; adjust pH 7.0.
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